Journal: Heliyon
Article Title: Biological changes in human B-cell line Ramos (RA.1) related to increasing doses of human parathyroid hormone
doi: 10.1016/j.heliyon.2024.e30556
Figure Lengend Snippet: Effects of PTH on B cells. A. Survival analyses of B cells stimulated for 48 h, histograms represent the average obtained from 5 experimental replicates B. Cellular proliferation of B cells stimulated at 24, 48, 72 and 96 h, histograms represent the proportion of the CFSE MFI read at time 2 over time 1, where T2 corresponds to the immediately greater time in which the reading was performed, data are representative of 5 experimental replicates C. IgM quantification in cell culture supernatant of B cells stimulated for 48 h, data are representative of 5 experimental replicates D, E. Effects of PTH on intracellular signaling in B cells stimulated for 4 h, protein levels were measured with Immunoblot and all data represent the mean and standard deviation of 5 experimental replicates. B cells from the Ramos cell line cultured without stimulus (a), CpG 0 .1 μM (b), CpG 0 .1 μM + PTH 0 .5 ng/mL (c), CpG 0 .1 μM + PTH 2 ng/mL (d) and CpG 0 .1 μM + PTH 8 ng/mL (e). Two-factor ANOVA and Dunnett's multiple comparison were applied to A and B, while Tukey's multiple comparison was applied to C and D. Significant values of p<0 . 05 were considered. Supplementary Material 1 includes the raw data of Lyn and its associated β-actin, and Supplementary Material 2 includes the raw data of pLyn and its associated β-actin. The bands 4–8 in both images correspond to conditions a-e respectively.
Article Snippet: The human B-cell line Ramos (RA.1) isolated from a Burkitt lymphoma of a 3-year-old male, was obtained from an ATCC supplier (Manassas, VA, USA; CRL-1596, Lot number: 70016960, CVCL_0597).
Techniques: Cell Culture, Western Blot, Standard Deviation, Comparison
ATCC is a verified supplier 
ATCC manufactures this product 
